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Literature summary for 3.1.4.11 extracted from

  • Rodriguez, R.; Matsuda, M.; Perisic, O.; Bravo, J.; Paul, A.; Jones, N.P.; Light, Y.; Swann, K.; Williams, R.L.; Katan, M.
    Tyrosine residues in phospholipase Cgamma2 essential for the enzyme function in B-cell signaling (2001), J. Biol. Chem., 276, 47982-47992.
    View publication on PubMed
Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant PLCgamma2, expression in DT40 cells, A431 cells and in Sf9 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
T753F PLCgamma2 mutant Homo sapiens
T753F/T759F active PLCgamma2 double mutant with a loss of tyrosine phosphorylation Homo sapiens
T759F PLCgamma2 mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ PLCgamma2, Ca2+-dependent, highest activity at 0.005-0.01 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16885 PLCgamma2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein stimulation of PLCgamma2 activity by tyrosine phosphorylation, multiple phosphorylation sites, the kinase Btk phosphorylates recombinant PLCgamma2 in vitro, Tyr-753 and Tyr-759 are essential for PLCgamma2 function in B-cells, preferential phosphorylation of Tyr-753 over Tyr-759 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant PLCgamma2, expressed in Sf9 cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Homo sapiens 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
additional information PLCgamma isoforms are regulated through activation of tyrosine kinase-linked receptors, importance of PLCgamma2 in B-cell signal transduction Homo sapiens ?
-
 ?