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Literature summary for 3.1.4.11 extracted from

  • Otterhag, L.; Sommarin, M.; Pical, C.
    N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana (2001), FEBS Lett., 497, 165-170.
    View publication on PubMed
Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment) Organism
AtPLC2, full-length and deletion variants, expression in Escherichia coli BL21(DE3)pLys Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information analysis of N-terminal deletion mutants of AtPLC2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane predominantly localized in Arabidopsis thaliana 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ AtPLC2 requires low micromolar levels of Ca2+, maximal phosphatidylinositol 4,5-bisphosphate hydrolysis at 0.001 mM free Ca2+, hydrolysis of phosphatidylinositol requires higher Ca2+ concentrations Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66142
-
 x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence Arabidopsis thaliana
95000
-
 x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana AtPLC2 is constitutively expressed ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39033 4 weeks old, AtPLC2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AtPLC2 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower AtPLC2 expression Arabidopsis thaliana
-
leaf AtPLC2 expression Arabidopsis thaliana
-
shoot AtPLC2 expression Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 60-350times higher specific activity with phosphatidylinositol 4,5-bisphosphate than with phosphatidylinositol as substrate Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O AtPLC2, preferred substrate, N-terminal EF-hand-like domain is required for catalytic activity but not for lipid binding Arabidopsis thaliana 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
additional information AtPLC2 is constitutively expressed Arabidopsis thaliana ?
-
 ?
phosphatidylinositol + H2O 60-350times lower specific activity than with phosphatidylinositol 4,5-bisphosphate Arabidopsis thaliana myo-inositol 1-phosphate + diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence Arabidopsis thaliana