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Literature summary for 3.1.4.11 extracted from
- Regulation of phospholipase C-beta1 activity by phosphatidic acid (2000), Biochemistry, 39, 7736-7743.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diacylglycerol | little activating effect on PLC-beta1 | Bos taurus |  |
| lysophosphatidic acid | slight activation, less effective in stimulating PLC-beta1 activity than phosphatidic acid | Bos taurus | |
| additional information | PLC-beta1 is not activated by phosphatidylserine, anionic and neutral detergents | Bos taurus | |
| oleic acid | little activating effect on PLC-beta1 | Bos taurus | |
| phosphatidic acid | PLC-beta1 activation, Ca2+-dependent, relatively independent of acyl chain length, stimulates basal and receptor-G protein-regulated PLC-beta1 activity, activation occurs through an C-terminal-dependent and an independent mechanism | Bos taurus | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | C-terminal deletion mutant: 100 kDa fragment with catalytic activity | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cholic acid | inhibits PLC-beta1 activity | Bos taurus | |
| deoxycholate | 0.1 mM, inhibits basal and phosphatidic acid-stimulated PLC-beta1 activity | Bos taurus | |
| phosphatidylserine | 30 mol%, slight PLC-beta1 inhibition | Bos taurus | |
| Triton X-100 | inhibits PLC-beta1 activity | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | pH 7, PLC-beta1 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | PLC-beta1 | Bos taurus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement, phosphatidic acid reduces the Ca2+ concentration required for half maximal stimulation of PLC-beta1 activity | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
PLC-beta1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PLC-beta1 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | PLC-beta1 | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | PLC-beta1 | Bos taurus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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