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Literature summary for 3.1.4.11 extracted from

  • Roy, G.; Villar, L.M.; Lazaro, I.; Gonzalez, M.; Bootello, A.; Gonzalez-Porque, P.
    Purification and properties of membrane and cytosolic phosphatidylinositol-specific phospholipase C from human spleen (1991), J. Biol. Chem., 266, 11495-11501.
    View publication on PubMed
Search for more literature for 3.1.4.11

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ concentrations above 1 mM are inhibitory Homo sapiens
EDTA inhibition by EDTA can be reverted upon the addition of increasing concentrations of Ca2+ and Mg2+ in the assay Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm Homo sapiens 5829
-
membrane PI-PLCm represents the main activity detected in the membrane, while PI-PLCc is the main activity present in the cytoplasm Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 high salt concentration, (NH4)2SO4, 2 M, dissociates the high molecular weight form yielding the low molecular form and increasing the specific activity Homo sapiens
Ca2+ PIPLC (cytosol) presents a total dependence on the presence of Ca2+ in the assay, which cannot be substituted by other divalent cations (Mg2+, Mn2+, Zn2+) Homo sapiens
Mg2+ no requirement for the membrane enzyme solubilized with sodium cholate, activates the membrane enzyme after solubilization in octyl glucoside with maximal activity at 0.1 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 PI-PLC, can be resolved into two peaks of activity of high M (60000-70000) and low M (16000-18000) Homo sapiens
18000
-
 purified Pi-PLC (membrane) has a molecular weight of about 18000, SDS-PAGE or gel filtration Homo sapiens
57000
-
 purified Pi-PLC (cytosol), SDS-PAGE or gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-acyl-2-arachidonoylphosphatidylinositol + H2O
-
 Homo sapiens ?
-
 ?
1-acyl-2-linoleoylphosphatidylinositol + H2O
-
 Homo sapiens ?
-
 ?
additional information no phospholipase A2 activity is found in the purified enzymes Homo sapiens ?
-
 ?
phosphatidylinositol + H2O Pi-PLC (membrane) and Pi-PLC (cytosol) are specific for this substrate Homo sapiens diacylglycerol + 1D-myo-inositol 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
low molecular weight PI-PLC
-
 Homo sapiens
phosphatidylinositol-specific phospholipases C
-
 Homo sapiens
PI-PLC
-
 Homo sapiens
PI-PLCc
-
 Homo sapiens
PI-PLCm
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 Pi-PLC (cytosol) Homo sapiens
7
-
 Pi-PLC (membrane) solubilized with sodium cholate Homo sapiens
7.8
-
 assay at Homo sapiens
8
-
 Pi-PLC (membrane) solubilized with octyl glucoside Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 both PI-PLC (membrane) and PI-PLC (cytosol) are fairly stable proteins when incubated for short periods of time (30 min) to a wide range of pH. PI-PLC (membrane) shows a fall of about 20-30% activity below pH 5.0 and is stable to alkaline pH up to 11 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens purified Pi-PLC (cytosol)
-
 6.2
Homo sapiens purified Pi-PLC (membrane) 9.2 9