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Literature summary for 3.1.4.11 extracted from

  • Flick, J.S.; Thorner, J.
    Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae (1993), Mol. Cell. Biol., 13, 5861-5876.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.11

Protein Variants

Protein Variants Comment Organism
additional information cells carrying either of two different deletion-insertion mutations, plcIDELTA1::HIS3 and plcDELTA2::LEU2, are viable but display several distinctive phenotypes, including temperature-sensitive growth, inviable above 35°C, osmotic sensitivity and defects in utilization of galactose, raffinose and glycerol at permissive temperatures, 23°C to 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100600
-
 x * 100600, calculation from nucleotide sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylinositol 4,5-bisphosphate + H2O Saccharomyces cerevisiae the enzyme generates two second messengers: D-myo-inositol 1,4,5-trisphosphate and diacylglycerol D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol + H2O Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 4,5-bisphosphate + H2O
-
 Saccharomyces cerevisiae D-myo-inositol 1,4,5-trisphosphate + diacylglycerol Ca2+ required. At 0.1 mM the enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate at faster rate than phosphatidylinositol, at 0.005 mM Ca2+ it hydrolyzes phosphatidylinositol 4,5-diphosphate exclusively ?
phosphatidylinositol 4,5-bisphosphate + H2O the enzyme generates two second messengers: D-myo-inositol 1,4,5-trisphosphate and diacylglycerol Saccharomyces cerevisiae D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 100600, calculation from nucleotide sequence Saccharomyces cerevisiae