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Literature summary for 3.1.31.1 extracted from
- Gene- and protein-delivered zinc finger-staphylococcal nuclease hybrid for inhibition of DNA replication of human papillomavirus (2013), PLoS ONE, 8, e56633.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient replication of HPV-18 mutant ori plasmids with gene-delivered chimeric mutant AZP-SNase, overexpression of recombinant mutant chimeric enzyme, hybrid nuclease AZP-SNase, in Escherichia coli | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the enzyme is fused in a chimeric protein to artificial zinc-finger protein, which inhibits virus DNA replication in planta and in 293H cells by blocking binding of a viral replication protein to its replication origin. The resulting hybrid nuclease AZP-SNase cleaves its target DNA plasmid efficiently and sequence-specifically in vitro, and expressed in cells, it inhibits human papillomavirus HPV-18 DNA replication cleaving an HPV-18 ori plasmid around its binding site, overview | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SNase | - |
Staphylococcus aureus |
| staphylococcal nuclease | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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