Protein Variants | Comment | Organism |
---|---|---|
F61W/W140A | the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme | Staphylococcus sp. |
W140A | the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme | Staphylococcus sp. |
Y93W/W140A | the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme | Staphylococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics for wild-type SNase and its mutants, overview | Staphylococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
gene nuc | - |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure of SNase and its mutants, W140A and Y93W/W140A have less alpha-helical content than the wild-type, overview | Staphylococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus sp. |
staphylococcal nuclease | - |
Staphylococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Staphylococcus sp. |
General Information | Comment | Organism |
---|---|---|
additional information | residue W140 is critical to SNase structure and function | Staphylococcus sp. |