Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.31.1 extracted from

  • Hu, H.Y.; Wu, M.C.; Fang, H.J.; Forrest, M.D.; Hu, C.K.; Tsong, T.Y.; Chen, H.M.
    The role of tryptophan in staphylococcal nuclease stability (2010), Biophys. Chem., 151, 170-177.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F61W/W140A the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme Staphylococcus sp.
W140A the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme Staphylococcus sp.
Y93W/W140A the mutant shows reduced activity with higher Michaelis-Menten constants, Km, and lower maximum reaction rate compared to the wild-type enzyme, the mutant also shows a more rapid loss of secondary and tertiary structure by Gdn-HCl unfolding than the wild-type enzyme Staphylococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics for wild-type SNase and its mutants, overview Staphylococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
gene nuc
-

Subunits

Subunits Comment Organism
More secondary structure of SNase and its mutants, W140A and Y93W/W140A have less alpha-helical content than the wild-type, overview Staphylococcus sp.

Synonyms

Synonyms Comment Organism
SNase
-
Staphylococcus sp.
staphylococcal nuclease
-
Staphylococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
assay at Staphylococcus sp.

General Information

General Information Comment Organism
additional information residue W140 is critical to SNase structure and function Staphylococcus sp.