Cloned (Comment) | Organism |
---|---|
a hyperstable, acid-resistant form of SNase known as delta+PHS is expressed in Escherichia coli BL21/DE3 cells | Staphylococcus sp. |
Crystallization (Comment) | Organism |
---|---|
crystals of the hyperstable mutant enzyme delta+PHS are grown using hanging drop vapor-diffusion methods at 4°C from a solution containing 17% 2-methyl-2,4-pentanediol, 2 M CaCl2, 3 M thymine-3',5'-diphosphate, and 25 mM potassium phosphate buffer, pH 8.0 | Staphylococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
G50F/V51N/P117G/H124L/S128A/DELTA44-49 | hyperstable, acid-resistant mutant form of enzyme known as delta+PHS | Staphylococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the carboxylic groups in the active site of SNase are known to bind Ca2+ | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus sp. |
staphylococcal nuclease | - |
Staphylococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | the overall secondary and tertiary structure of SNase remains unchanged between pH 2.5 and 8.0 | Staphylococcus sp. |