Cloned (Comment) | Organism |
---|---|
wild type and mutant Snases are expressed in Escherichia coli BL-21 (DE3) as inclusion bodies | Staphylococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
Y54C/I139C | production by site-directed mutagenesis, the oxidized form assumes a more compact denatured structure under acidic conditions than the wild type, the kinetic measurements reveal that the refolding reactions of both the reduced and oxidized forms of mutant are similar to those of the wild type protein | Staphylococcus sp. |
Y54C/I139C/DELTA140-149 | production by site-directed mutagenesis, under physiological conditions, the reduced form appears to assume a denatured structure, in contrast, the oxidized form forms a native-like structure | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzyme are purified by a series of urea extraction, ethanol precipitation, and ion exchange chromatography | Staphylococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus sp. |
staphylococcal nuclease | - |
Staphylococcus sp. |