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Literature summary for 3.1.31.1 extracted from

  • Volkening, J.D.; Spatz, S.J.
    Purification of DNA from the cell-associated herpesvirus Mareks disease virus for 454 pyrosequencing using micrococcal nuclease digestion and polyethylene glycol precipitation (2009), J. Virol. Methods, 157, 55-61.
    View publication on PubMed

Application

Application Comment Organism
analysis purification of DNA from the cell-associated herpesvirus Marekย’s disease virus Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
purification of DNA from the cell-associated herpesvirus Marek's disease virus. 150 U of Micrococcal nuclease added to gallid herpesvirus type 2 virus infected cells (GaHV-2 strain 648A) in 100 microl reaction volume, digestion followed by PEG precipitation yields high-molecular weight DNA of greater than 75% pure GaHV-2 DNA suitability for both direct pyrosequencing and further amplification using isothermal polymerase Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Staphylococcus aureus
-
3'-deoxymononucleotides + dinucleotides
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
?

Synonyms

Synonyms Comment Organism
Micrococcal nuclease
-
Staphylococcus aureus