Protein Variants | Comment | Organism |
---|---|---|
A1C/Q149C | SNase double mutant, N- and C-terminal residues replaced by cysteine, constructed from the plasmid (pMT7-SN) of wild-type SNase using the Kunkel method, can form disulfide bond | Staphylococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxythymidine 3',5'-diphosphate | in the presence of pdTp, unfolding forces increase drastically | Staphylococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, unfolding forces in the presence of calcium ion are slightly increased | Staphylococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Staphylococcus sp. | - |
3'-deoxymononucleotides + dinucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
10 mM DTT added to expose sulfhydryl groups, and the solution passes through a short column of PD-10 to remove the reducing agent | Staphylococcus sp. |
Renatured (Comment) | Organism |
---|---|
atomic force microscopy, unfolding experiment: multiplicity of unfolding intermediates of SNase at the single-molecule level, mechanical unfolding pathways can be changed drastically under the acid-denatured condition (native conditions: 1 mM EGTA, pH 8.0, acid-denaturated condition: 1 mM EGTA, pH 2.5) or in the presence of ligands (Ca2+) and inhibitors (2'-deoxythymidine 3',5'-diphosphate) | Staphylococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Staphylococcus sp. | 3'-deoxymononucleotides + dinucleotides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | small globular protein (149 amino acid residues), native protein: no disulfide bond, double mutant (A1C/Q149C): can form disulfide bond | Staphylococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus sp. |
staphylococcal nuclease | - |
Staphylococcus sp. |