Cloned (Comment) | Organism |
---|---|
generation of plasmids pSS and pDS (for induction of suicide mechanism) consisting of kanamycin resistance, T7-promotor, Serratia marcescens nuclease (1 copy in pSS, 2 copies in pDS) minus its lead-peptide-coding nucleotide sequence. cotransformation with plasmid pL-EGFP-OPH (containing organophosphorus hydrolase and enhanced green fluorescent protein for degradation of organophosphates) of Escherichia coli strain BL21-AI(TM) to generate BL21AI-GOS (pDS) | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Serratia marcescens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cells transformed with plasmid pDS (carrying 2 copies of Serratia marcescens nuclease) are more effective in stopping cell division than those transformed with plasmid pSS (carrying 1 copy of Serratia marcescens nuclease), OD600 analysis, colony test on plates. Cells cotransformed grow more slowly before induction of Serratia marcescens nuclease, OD600 analysis, florescence, colony test on plates | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Serratia marcescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Serratia marcescens nuclease | - |
Serratia marcescens |