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Literature summary for 3.1.3.86 extracted from

  • Woscholski, R.; Waterfield, M.D.; Parker, P.J.
    Purification and biochemical characterization of a mammalian phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase (1995), J. Biol. Chem., 270, 31001-31007.
    View publication on PubMed
Search for more literature for 3.1.3.86

Inhibitors

Inhibitors Comment Organism Structure
inositol hexakissulfate inhibitory at high concentrations Rattus norvegicus
additional information N-ethylmaleimide, orthophosphate, 4-nitrophenylphosphate, glycerolbisphosphate, inositol hexakisphosphate and (1R,2R,4R)-cyclohexane-1,2,4-trismethylenesulfonate are without significant effect on the enzyme activity Rattus norvegicus
neomycin partial inhibition Rattus norvegicus
phosphatidylinositol 4,5-bisphosphate strong inhibition at low micromolar concentrations Rattus norvegicus
phosphatidylinositol 4-phosphate inhibition at low micromolar concentrations Rattus norvegicus
vanadate partial inhibition at millimolar concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication Rattus norvegicus
0.1
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Rattus norvegicus
additional information Zn2+, Ca2+ and Mn2+ do not support enzyme activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap S column chromatography, phosphocellulose column chromatography, heparin-HiTrap column chromatography, Q-HiTrap column chromatography, and S-300 gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
 Rattus norvegicus 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Rattus norvegicus 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
-
 Rattus norvegicus
PtdIns(3,4,5)P3 5-phosphatase
-
 Rattus norvegicus
SHIP
-
 Rattus norvegicus