Literature summary for 3.1.3.74 extracted from

Kim, J.E.; Kim, D.W.; Kwak, S.E.; Ryu, H.J.; Yeo, S.I.; Kwon, O.S.; Choi, S.Y.; Kang, T.C.
Pyridoxal-5-phosphate phosphatase/chronophin inhibits long-term potentiation induction in the rat dentate gyrus (2009), Hippocampus, 19, 1078-1089.

Cloned(Commentary)

Cloned (Comment)	Organism
getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). investigation whether expression of PLPP/CIN is altered following long-term potentiation (LTP) induction and whether Tat-PLPP/CIN transduction affects LTP induction in the rat Sprague-Dawley. PLPP/CIN immunoreactivity is decreased in dentate granule cells after induction of LTP (induction: stimulating electrode for high frequency stimulation and recording electrode). Tat-PLPP/CIN transduction (20 and 200 microg/kg) decreased the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude as compared to saline or Tat-protein-treated animals. PLPP/CIN protein level shows inverse correlation with phosphorylated ADF/cofilin levels and F-actin content (immunohistochemistry). conclusion: PLPP/CIN-mediated actin dynamics may play important role in changes of morphological properties (dendritic spine reorganization) of the hippocampus in LTP.	Rattus norvegicus

Cloned (Comment)

Organism

getting Tat-PLPP/CIN: amplification of PLPP/CIN via PCR and inserted into pGEM-T vector, subcloned into pET15b, transformation of BL21 Escherichia coli. getting PLPP: cDNA of PLPP amplified via PCR and cloned into pET15b, transformation of Escherichia coli JM109 (DE3). investigation whether expression of PLPP/CIN is altered following long-term potentiation (LTP) induction and whether Tat-PLPP/CIN transduction affects LTP induction in the rat Sprague-Dawley. PLPP/CIN immunoreactivity is decreased in dentate granule cells after induction of LTP (induction: stimulating electrode for high frequency stimulation and recording electrode). Tat-PLPP/CIN transduction (20 and 200 microg/kg) decreased the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude as compared to saline or Tat-protein-treated animals. PLPP/CIN protein level shows inverse correlation with phosphorylated ADF/cofilin levels and F-actin content (immunohistochemistry). conclusion: PLPP/CIN-mediated actin dynamics may play important role in changes of morphological properties (dendritic spine reorganization) of the hippocampus in LTP.

Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	directly dephosphorylates actin-depolymerizing factor (ADF)/cofilin	?	-	?
pyridoxal 5'-phosphate + H2O	Rattus norvegicus	-	pyridoxal + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	Sprague-Dawley rat	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
dentate gyrus	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	21.3 units, Tat-protein transduction (200 microg/kg), long-term potentiation induced by high frequency stimulation (2000 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	22.3 units, control animals (saline-treated and without high-frequency stimulation), long-term potentiation induced by high frequency stimulation (2000 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	26.1 units, control animals (saline-treated and without high-frequency stimulation), long-term potentiation induced by high frequency stimulation (1600 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	27.8 units, Tat-protein transduction (200 microg/kg), long-term potentiation induced by high frequency stimulation (1600 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	29.1 units, Tat-protein transduction (200 microg/kg), long-term potentiation induced by high frequency stimulation (1200 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	29.4 units, control animals (saline-treated and without high-frequency stimulation), long-term potentiation induced by high frequency stimulation (1200 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	32.1 units, control animals (saline-treated and without high-frequency stimulation), long-term potentiation induced by high frequency stimulation (800 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	33.7 units, Tat-protein transduction (200 microg/kg), long-term potentiation induced by high frequency stimulation (800 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	42.1 units, control animals (saline-treated and without high-frequency stimulation), no long-term potentiation induced, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	43.2 units, Tat-protein transduction (200 microg/kg), no long-term potentiation induced, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	59.8 units, Tat Tat-PLPP/CIN transductions (20 microg/kg), long-term potentiation induced by high frequency stimulation (1600 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	60.4 units, Tat-PLPP/CIN transductions (20 microg/kg), long-term potentiation induced by high frequency stimulation (800 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	62.3 units, Tat-PLPP/CIN transductions (20 microg/kg), long-term potentiation induced by high frequency stimulation (2000 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	62.3 units, Tat-PLPP/CIN transductions (20 microg/kg), no long-term potentiation induced, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	68.9 units, Tat-PLPP/CIN transductions (20 microg/kg), long-term potentiation induced by high frequency stimulation (1200 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	71.0 units, Tat-PLPP/CIN transductions (200 microg/kg), no long-term potentiation induced, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	74.2 units, Tat-PLPP/CIN transductions (200 microg/kg), long-term potentiation induced by high frequency stimulation (1200 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	80.6 units, Tat-PLPP/CIN transductions (200 microg/kg), long-term potentiation induced by high frequency stimulation (800 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	82.3 units, Tat Tat-PLPP/CIN transductions (200 microg/kg), long-term potentiation induced by high frequency stimulation (1600 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus
additional information	-	85.4 units, Tat-PLPP/CIN transductions (200 microg/kg), long-term potentiation induced by high frequency stimulation (2000 total stimuli, delivered in two five 1 s long tetanic (400 Hz) stimulus trains, 2 min apart), pH 7.4 in 40 mM triethanolamineamine-HCl, measuring the rate of production of pyridoxal from pyridoxal-5'-phosphate, absorbance at 390 nm (3 min)	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	directly dephosphorylates actin-depolymerizing factor (ADF)/cofilin	Rattus norvegicus	?	-	?
pyridoxal 5'-phosphate + H2O	-	Rattus norvegicus	pyridoxal + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PLPP/CIN	pyridoxal-5'-phosphate (PLP)-phosphatase/chronophin	Rattus norvegicus