Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Legionella pneumophila |
Crystallization (Comment) | Organism |
---|---|
crystal structures of SidF and its complex with bound short-chain (dibutanoyl) derivative of PI(3,4)P2 is shown. The structures show that the conserved CX5R catalytic motif is located in a large cationic groove | Legionella pneumophila |
Protein Variants | Comment | Organism |
---|---|---|
C645S | inactive mutant | Legionella pneumophila |
additional information | deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery | Legionella pneumophila |
additional information | deletion of the putative transmembrane domains does not affect its enzymatic activity | Legionella pneumophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | ectopically expressed GFP-SidF localizes to the ER membrane in mammalian cells. Deletion of the C-terminal portion of SidF including the two transmembrane motifs changes its localization to the cell periphery | Legionella pneumophila | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | Q5ZSD5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol 3,4,5-trisphosphate + H2O | - |
Legionella pneumophila | phosphatidylinositol 4,5-bisphosphate + phosphate | - |
? | |
phosphatidylinositol 3,4-bisphosphate + H2O | preferred substrate | Legionella pneumophila | phosphatidylinositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SidF | - |
Legionella pneumophila |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of SidF results in the reduced recruitment of effector proteins that anchor on the Legionella-containing vacuole (LCV) via binding to PI(4)P | Legionella pneumophila |