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Literature summary for 3.1.3.67 extracted from

  • Feng, W.; Wu, H.; Chan, L.N.; Zhang, M.
    Par-3-mediated junctional localization of the lipid phosphatase PTEN is required for cell polarity establishment (2008), J. Biol. Chem., 283, 23440-23449.
    View publication on PubMed
Search for more literature for 3.1.3.67

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal domain of enzyme is expressed in BL21 (DE3) Escherichia coli cells as glutathione S-transferase-protein. Par-3/PDZ3-PTEN peptide single chain fusion protein contains a thrombin cleavage site (LVPRGS) between the C-terminus of PDZ3 domain and the PTEN peptide (DEDQSHQITKV), production of a canine knockdown Canis lupus

Protein Variants

Protein Variants Comment Organism
DELTA394-403 removal of the last 10 residues of PTEN leads to the complete diffusion of PTEN in MDCK cells, failing to restore the junctional localization of phosphatidylinositol 4,5-bisphosphate Canis lupus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Canis lupus the Par-3/PDZ3-PTEN binding is required for the enrichment of PTEN at the junctional membranes of Madin-Darby canine kidney cells, the junctional membrane-localized PTEN is specifically required for the polarization of Madin-Darby canine kidney cells ?
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 ?
additional information Canis lupus the regulatory function of PTEN in cell polarity is specifically mediated by its interaction with Par-3, the junctional membrane localization of PTEN depends on this specific Par-3/PDZ3-PTEN tail peptide interaction ?
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 ?

Organism

Organism UniProt Comment Textmining
Canis lupus
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Purification (Commentary)

Purification (Comment) Organism
fusion-protein is purified by Glutathione-Sepharose-4B affinity chromatography Canis lupus

Source Tissue

Source Tissue Comment Organism Textmining
MDCK cell Madin-darby canine kidney cell Canis lupus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the Par-3/PDZ3-PTEN binding is required for the enrichment of PTEN at the junctional membranes of Madin-Darby canine kidney cells, the junctional membrane-localized PTEN is specifically required for the polarization of Madin-Darby canine kidney cells Canis lupus ?
-
 ?
additional information the regulatory function of PTEN in cell polarity is specifically mediated by its interaction with Par-3, the junctional membrane localization of PTEN depends on this specific Par-3/PDZ3-PTEN tail peptide interaction Canis lupus ?
-
 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 3,4,5-trisphosphate-specific phosphatase
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 Canis lupus
PTEN
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 Canis lupus