Application | Comment | Organism |
---|---|---|
analysis | availability of the phosphorylated and unphosphorylated forms of recombinant PTEN permits future investigations into the three-dimensional structures of the phosphorylated and unphosphorylated forms of PTEN, and the role of phosphorylation in regulating PTEN activity, phospholipid- and protein-binding affinities | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
PTEN is ligated into the pBacPAK9 baculovirus transfer vector. Sf9 cells are infected with the recombinant PTEN baculovirus | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
50-53 kDa, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | O54857 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | recombinant PTEN is phosphorylated in the infected insect cells at Ser-380, Thr-382, and Thr-383 at the C-terminal tail | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant PTEN is purified to near homogeneity using four sequential column chromatographic steps: a diethylaminoethyl (DEAE) Sepharose anion exchange column, a bio-gel hydroxyapatite HT (HAP) column, a Mono-S cation exchange column, and a Mono-Q anion exchange column | Rattus norvegicus |
treatment with alkaline phosphatase fully dephosphorylates the phosphorylation sites. Unphosphorylated PTEN and alkaline phosphatase can be separated by ion exchange column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myeloma cell | amplified by PCR from | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol 3,4,5-trisphosphate + H2O | - |
Rattus norvegicus | phosphatidyl inositol 4,5-bisphosphate + phosphate | - |
? | |
phosphatidylinositol-3,4-bisphosphate + H2O | - |
Rattus norvegicus | phosphatidylinositol-4-monophosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTEN | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |