Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.67 extracted from

  • Gajewski, J.E.; Bird, M.J.; Crowhurst, M.O.; Sio-Seng Lio, D.; Liu, J.; Wettenhall, R.E.; Zhu, H.J.; Cheng, H.C.
    Expression, generation, and purification of unphosphorylated and phospho-Ser-380/Thr-382/Thr-383 form of recombinant PTEN phosphatase (2007), Protein Expr. Purif., 55, 334-342.
    View publication on PubMed

Application

Application Comment Organism
analysis availability of the phosphorylated and unphosphorylated forms of recombinant PTEN permits future investigations into the three-dimensional structures of the phosphorylated and unphosphorylated forms of PTEN, and the role of phosphorylation in regulating PTEN activity, phospholipid- and protein-binding affinities Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
PTEN is ligated into the pBacPAK9 baculovirus transfer vector. Sf9 cells are infected with the recombinant PTEN baculovirus Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
50-53 kDa, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus O54857
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein recombinant PTEN is phosphorylated in the infected insect cells at Ser-380, Thr-382, and Thr-383 at the C-terminal tail Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
recombinant PTEN is purified to near homogeneity using four sequential column chromatographic steps: a diethylaminoethyl (DEAE) Sepharose anion exchange column, a bio-gel hydroxyapatite HT (HAP) column, a Mono-S cation exchange column, and a Mono-Q anion exchange column Rattus norvegicus
treatment with alkaline phosphatase fully dephosphorylates the phosphorylation sites. Unphosphorylated PTEN and alkaline phosphatase can be separated by ion exchange column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
myeloma cell amplified by PCR from Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol 3,4,5-trisphosphate + H2O
-
Rattus norvegicus phosphatidyl inositol 4,5-bisphosphate + phosphate
-
?
phosphatidylinositol-3,4-bisphosphate + H2O
-
Rattus norvegicus phosphatidylinositol-4-monophosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
PTEN
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rattus norvegicus