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Literature summary for 3.1.3.67 extracted from

  • Kabuyama, Y.; Nakatsu, N.; Homma, Y.; Fukui, Y.
    Purification and characterization of the phosphatidylinositol-3,4,5-trisphosphate phosphatase in bovine thymus (1996), Eur. J. Biochem., 238, 350-356.
    View publication on PubMed
Search for more literature for 3.1.3.67

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ in presence of 1 mM Mg2+, slight activation up to 0.01 mM, significant inhibition above 0.1 mM Bos taurus
Mg2+ divalent cation required, maximal activition at 1 mM, inhibition above 1 mM Bos taurus
phosphatidylserine 0.2 mM, 75% loss of activity Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in presence of 1 mM Mg2+, slight activation up to 0.01 mM, significant inhibition above 0.1 mM Bos taurus
Mg2+ divalent cation required, maximal activition at 1 mM, inhibition above 1 mM Bos taurus
Mn2+ divalent cation required, Mn2+ is less effective than Mg2+. 0.5 mM Mn2+ gives 30% of the maximal activity with 1 mM Mg2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphatidylinositol-3,4,5-trisphosphate + H2O Bos taurus the enzyme may play a critical role in the inositolphospholipid 3-kinase signalling ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not hydrolyze phosphatidylinositol 4,5-bisphosphate Bos taurus ?
-
 ?
Phosphatidylinositol-3,4,5-trisphosphate + H2O
-
 Bos taurus Phosphatidyl inositol-4,5-bisphosphate + phosphate
-
 ?
Phosphatidylinositol-3,4,5-trisphosphate + H2O the enzyme may play a critical role in the inositolphospholipid 3-kinase signalling Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 120000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bos taurus