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Literature summary for 3.1.3.62 extracted from

  • Caffrey, J.J.; Hidaka, K.; Matsuda, M.; Hirata, M.; Shears, S.B.
    The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification (1999), FEBS Lett., 442, 99-104.
    View publication on PubMed
Search for more literature for 3.1.3.62

Cloned(Commentary)

Cloned (Comment) Organism
MIPP cDNA from Jurkat T-cells is cloned and expressed in Escherichia coli M15 without the ER-targeting domain, full-length sequence Homo sapiens
MIPP cDNA from liver and kidney is cloned and expressed in Escherichia coli M15, full-length sequence Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H370A mutant with less than 5% of activity of wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum ER-based protein, N-terminal ER-targeting domain Rattus norvegicus 5783
-
endoplasmic reticulum ER-based protein, N-terminal ER-targeting domain Homo sapiens 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+-independent Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis Homo sapiens
53400
-
 x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus MIPP mRNA is upregulated during chondrocyte hypertrophy, MIPP may aid bone mineralization and salvage the inositol moiety prior to apoptosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UNW1
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MIPP expressed in Escherichia coli M15 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
chondrocyte MIPP expression pattern in developing chondrocytes Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
kidney 2.5 kb mRNA expression Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver 2.5 kb mRNA expression Homo sapiens
-
additional information not expressed in peripheral blood leukocytes Homo sapiens
-
placenta 2.5 kb mRNA expression Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Rattus norvegicus D-myo-inositol 1,4,5-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4,5-trisphosphate + phosphate
-
 ?
inositol hexakisphosphate + H2O
-
 Rattus norvegicus inositol pentakisphosphate + phosphate
-
 ?
additional information dephosphorylates inositol signaling molecules InsP5 and InsP6 Rattus norvegicus ?
-
 ?
additional information catalytic requirement for the C-terminus and His-370 of MIPP Homo sapiens ?
-
 ?
additional information MIPP mRNA is upregulated during chondrocyte hypertrophy, MIPP may aid bone mineralization and salvage the inositol moiety prior to apoptosis Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53400, recombinant MIPP expressed in Escherichia coli M15, amino acid sequence analysis Homo sapiens
? x * 51000, recombinant MIPP expressed in Escherichia coli M15, SDS-PAGE and Western blot analysis Homo sapiens

Synonyms

Synonyms Comment Organism
More structural and functional relationship between MIPP and histidine acid phosphatase Rattus norvegicus
More structural and functional relationship between MIPP and histidine acid phosphatase Homo sapiens