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Literature summary for 3.1.3.5 extracted from
- Purification and properties of cytosol 5'-nucleotidase from black rockfish (Sebastes inermis) muscle (1993), J. Agric. Food Chem., 41, 1208-1212.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibition of cytosolic and membrane-bound enzyme | Sebastes inermis |  |
| benzoic acid | inhibition of cytosolic enzyme | Sebastes inermis | |
| L-ascorbic acid | inhibition of cytosolic enzyme | Sebastes inermis | |
| p-chloromercuribenzoate | inhibition of cytosolic and membrane-bound enzyme | Sebastes inermis | |
| phenylmethylsulfonyl fluoride | inhibition of cytosolic enzyme | Sebastes inermis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0307 | - |
5'-AMP | cytosolic enzyme | Sebastes inermis | |
| 0.0622 | - |
5'-IMP | cytosolic enzyme | Sebastes inermis | |
| 0.131 | - |
5'-UMP | cytosolic enzyme | Sebastes inermis | |
| 0.215 | - |
5'-GMP | cytosolic enzyme | Sebastes inermis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Sebastes inermis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates cytosolic and membrane-bound enzyme | Sebastes inermis | |
| Mn2+ | activates cytosolic and membrane-bound enzyme | Sebastes inermis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE | Sebastes inermis |
| 148000 | - |
1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE | Sebastes inermis |
| 250000 | - |
cytosolic enzyme, gel filtration | Sebastes inermis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sebastes inermis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Sebastes inermis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
cytosolic enzyme | Sebastes inermis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Sebastes inermis | adenosine + phosphate | - |
? | |
| 5'-GMP + H2O | - |
Sebastes inermis | guanosine + phosphate | - |
? | |
| 5'-IMP + H2O | - |
Sebastes inermis | inosine + phosphate | - |
? | |
| 5'-UMP + H2O | - |
Sebastes inermis | uridine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE | Sebastes inermis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
cytosolic enzyme stable for 30 min | Sebastes inermis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | cytosolic enzyme, 4°C, 16 h stable | Sebastes inermis |
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