Application | Comment | Organism |
---|---|---|
nutrition | the substrate 5'-IMP is an important commercial index of freshness of fish | Sebastes inermis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of cytosolic and membrane-bound enzyme | Sebastes inermis | |
EDTA | inhibition of membrane-bound enzyme | Sebastes inermis | |
p-chloromercuribenzoate | inhibition of cytosolic and membrane-bound enzyme | Sebastes inermis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
5'-AMP | membrane-bound enzyme | Sebastes inermis | |
0.061 | - |
5'-IMP | membrane-bound enzyme | Sebastes inermis | |
0.175 | - |
5'-UMP | membrane-bound enzyme | Sebastes inermis | |
0.178 | - |
5'-GMP | membrane-bound enzyme | Sebastes inermis | |
0.207 | - |
5'-CMP | membrane-bound enzyme | Sebastes inermis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Sebastes inermis | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates cytosolic and membrane-bound enzyme | Sebastes inermis | |
Mn2+ | activates cytosolic and membrane-bound enzyme | Sebastes inermis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
4 * 67000, membrane-bound enzyme, SDS-PAGE | Sebastes inermis |
265000 | - |
membrane-bound enzyme, gel filtration | Sebastes inermis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sebastes inermis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Sebastes inermis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
261 | - |
membrane-bound enzyme | Sebastes inermis |
Storage Stability | Organism |
---|---|
4°C, 40 mM Tris-HCl, 1 mM MgCl2, 110 mM NaCl, 1 mM CaCl2, 0.1% Triton X-100, 23% loss of activity in 5 months | Sebastes inermis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | - |
Sebastes inermis | adenosine + phosphate | - |
? | |
5'-CMP + H2O | - |
Sebastes inermis | cytidine + phosphate | - |
? | |
5'-GMP + H2O | - |
Sebastes inermis | guanosine + phosphate | - |
? | |
5'-IMP + H2O | - |
Sebastes inermis | inosine + phosphate | - |
? | |
5'-UMP + H2O | - |
Sebastes inermis | uridine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 67000, membrane-bound enzyme, SDS-PAGE | Sebastes inermis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
membrane-bound enzyme stable for 30 min | Sebastes inermis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
membrane-bound enzyme | Sebastes inermis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.8 | membrane-bound enzyme, 4°C, stable for 14 h | Sebastes inermis |