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Literature summary for 3.1.3.5 extracted from

  • Marseno, D.W.; Hori, K.; Miyazawa, K.
    Purification and properties of membrane-bound 5'-nucleotidase from black rockfish (Sebastes inermis) muscle (1993), J. Agric. Food Chem., 41, 863-869.
    View publication on PubMed

Application

Application Comment Organism
nutrition the substrate 5'-IMP is an important commercial index of freshness of fish Sebastes inermis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibition of cytosolic and membrane-bound enzyme Sebastes inermis
EDTA inhibition of membrane-bound enzyme Sebastes inermis
p-chloromercuribenzoate inhibition of cytosolic and membrane-bound enzyme Sebastes inermis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
5'-AMP membrane-bound enzyme Sebastes inermis
0.061
-
5'-IMP membrane-bound enzyme Sebastes inermis
0.175
-
5'-UMP membrane-bound enzyme Sebastes inermis
0.178
-
5'-GMP membrane-bound enzyme Sebastes inermis
0.207
-
5'-CMP membrane-bound enzyme Sebastes inermis

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Sebastes inermis 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates cytosolic and membrane-bound enzyme Sebastes inermis
Mn2+ activates cytosolic and membrane-bound enzyme Sebastes inermis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
4 * 67000, membrane-bound enzyme, SDS-PAGE Sebastes inermis
265000
-
membrane-bound enzyme, gel filtration Sebastes inermis

Organism

Organism UniProt Comment Textmining
Sebastes inermis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Sebastes inermis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
261
-
membrane-bound enzyme Sebastes inermis

Storage Stability

Storage Stability Organism
4°C, 40 mM Tris-HCl, 1 mM MgCl2, 110 mM NaCl, 1 mM CaCl2, 0.1% Triton X-100, 23% loss of activity in 5 months Sebastes inermis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
Sebastes inermis adenosine + phosphate
-
?
5'-CMP + H2O
-
Sebastes inermis cytidine + phosphate
-
?
5'-GMP + H2O
-
Sebastes inermis guanosine + phosphate
-
?
5'-IMP + H2O
-
Sebastes inermis inosine + phosphate
-
?
5'-UMP + H2O
-
Sebastes inermis uridine + phosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 67000, membrane-bound enzyme, SDS-PAGE Sebastes inermis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
membrane-bound enzyme stable for 30 min Sebastes inermis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
membrane-bound enzyme Sebastes inermis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 8.8 membrane-bound enzyme, 4°C, stable for 14 h Sebastes inermis