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Literature summary for 3.1.3.5 extracted from

  • Gupta, A.; Sharma, C.B.
    Purification to homogeneity and characterization of plasma membrane and Golgi apparatus-specific 5'-adenosine monophosphatases from peanut cotyledons (1996), Plant Sci., 117, 65-74.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
5'-AMP enzyme from plasma membrane Arachis hypogaea
1.02
-
5'-AMP enzyme from Golgi apparatus Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Arachis hypogaea 5794
-
plasma membrane
-
Arachis hypogaea 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea
52400
-
x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification enzymes from plasma membrane and Golgi apparatus have 37% and 39% carbohydrate, respectively Arachis hypogaea

Purification (Commentary)

Purification (Comment) Organism
enzyme from plasma membrane and enzyme from Golgi apparatus Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
182
-
enzyme from Golgi apparatus Arachis hypogaea
589.4
-
enzyme from plasma membrane Arachis hypogaea

Storage Stability

Storage Stability Organism
-20°C, 24 h, 80-90% loss of activity Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O best substrate for enzyme from plasma membrane and Golgi apparatus Arachis hypogaea adenosine + phosphate
-
?
5'-GMP + H2O plasma membrane enzyme: 2.85% of the activity with 5'-AMP, Golgi apparatus enzyme: 46% of the activity with 5'-AMP Arachis hypogaea guanosine + phosphate
-
?
5'-UMP + H2O plasma membrane enzyme: 7.5% of the activity with 5'-AMP, Golgi apparatus enzyme: 16.2% of the activity with 5'-AMP Arachis hypogaea uridine + phosphate
-
?
p-nitrophenylphosphate + H2O plasma membrane enzyme: 1% of the activity with 5'-AMP, Golgi apparatus enzyme: 39% of the activity with 5'-AMP Arachis hypogaea p-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 52400, enzyme from Golgi apparatus, x * 26300, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

Synonyms

Synonyms Comment Organism
GA-AMPase
-
Arachis hypogaea
PM-AMPase
-
Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 enzymes from plasma membrane and Golgi apparatus Arachis hypogaea