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Literature summary for 3.1.3.5 extracted from

  • Yamamoto, H.; Tomioka, K.; Kawai, H.; Endo, K.
    Purification and properties of 5'-nucleotidase from cod muscle (1986), Agric. Biol. Chem., 50, 1123-1129.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Gadus macrocephalus
ATP
-
Gadus macrocephalus
Cu2+
-
Gadus macrocephalus
EDTA
-
Gadus macrocephalus
Zn2+
-
Gadus macrocephalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
5'-AMP
-
Gadus macrocephalus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Gadus macrocephalus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
4 * 67000, SDS-PAGE Gadus macrocephalus
270000
-
gel filtration Gadus macrocephalus

Organism

Organism UniProt Comment Textmining
Gadus macrocephalus
-
cod
-

Purification (Commentary)

Purification (Comment) Organism
-
Gadus macrocephalus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Gadus macrocephalus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
828
-
-
Gadus macrocephalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O 101% of the activity of 5'-IMP Gadus macrocephalus adenosine + phosphate
-
?
5'-CMP + H2O 85% of the activity of 5'-IMP Gadus macrocephalus cytidine + phosphate
-
?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Gadus macrocephalus deoxyadenosine + phosphate
-
?
5'-dAMP + H2O 53% of the activity of 5'-IMP Gadus macrocephalus deoxyadenosine + phosphate
-
?
5'-GMP + H2O 52% of the activity of 5'-IMP Gadus macrocephalus guanosine + phosphate
-
?
5'-IMP + H2O equally reactive as 5'-AMP Gadus macrocephalus inosine + phosphate
-
?
5'-UMP + H2O 120% of the activity of 5'-IMP Gadus macrocephalus uridine + phosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 67000, SDS-PAGE Gadus macrocephalus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Gadus macrocephalus