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Literature summary for 3.1.3.5 extracted from

  • Sharma, C.B.; Mittal, R.; Tanner, W.
    Purification and properties of a glycoprotein adenosine 5'-monophosphatase from the plasma membrane fraction of Arachis hypogaea cotyledons (1986), Biochim. Biophys. Acta, 884, 567-577.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Arachis hypogaea
NaF
-
Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.08
-
5'-AMP
-
Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Arachis hypogaea 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification 42.7% carbohydrate, plasma membrane enzyme Arachis hypogaea

Purification (Commentary)

Purification (Comment) Organism
-
Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
700
-
-
Arachis hypogaea

Storage Stability

Storage Stability Organism
-20°C, purified enzyme inactivated in 24 h, membrane-bound enzyme stable for 2 weeks Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O highly specific, no hydrolysis of other nucleotides Arachis hypogaea adenosine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 55000, plasma membrane enzyme, SDS-PAGE Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Arachis hypogaea