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Literature summary for 3.1.3.5 extracted from
- Purification and characterization of membrane-bound 5'-nucleotidase of Vibrio parahaemolyticus (1989), J. Biochem., 105, 785-789.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| azolectin | stimulation | Vibrio parahaemolyticus |  |
General Stability
| General Stability | Organism |
|---|---|
| inactivation by repeated freezing and thawing | Vibrio parahaemolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | - |
Vibrio parahaemolyticus | |
| Zn2+ | - |
Vibrio parahaemolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | the catalytic site is oriented outward | Vibrio parahaemolyticus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Br- | can substitute for Cl- in activation | Vibrio parahaemolyticus | |
| Cl- | 20 mM optimal for activity | Vibrio parahaemolyticus | |
| Co2+ | 40% as effective as Mg2+ | Vibrio parahaemolyticus | |
| Mg2+ | dependent on | Vibrio parahaemolyticus | |
| Mn2+ | 40% as effective as Mg2+ | Vibrio parahaemolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
x * 72000, SDS-PAGE | Vibrio parahaemolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio parahaemolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio parahaemolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1400 | - |
- |
Vibrio parahaemolyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified enzyme, stable for at least 6 months | Vibrio parahaemolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Vibrio parahaemolyticus | adenosine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, SDS-PAGE | Vibrio parahaemolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Vibrio parahaemolyticus |
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