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Literature summary for 3.1.3.5 extracted from

  • Santos, C.A.; Saraiva, A.M.; Toledo, M.A.; Beloti, L.L.; Crucello, A.; Favaro, M.T.; Horta, M.A.; Santiago, A.S.; Mendes, J.S.; Souza, A.A.; Souza, A.P.
    Initial biochemical and functional characterization of a 5-nucleotidase from Xylella fastidiosa related to the human cytosolic 5-nucleotidase I (2013), Microb. Pathog., 59-60, 1-6.
    View publication on PubMed
Search for more literature for 3.1.3.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.224
-
 4-nitrophenyl phosphate pH 7.0, 22°C Xylella fastidiosa

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mM, 2fold activation Xylella fastidiosa
Cu2+ 0.1 mM, 1.5fold activation Xylella fastidiosa
Mg2+ 1 mM, 4fold increase in activity Xylella fastidiosa
Mn2+ 0.1 mM, 3.5fold activation Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
 2 * 35900, SDS-PAGE Xylella fastidiosa
74300
-
 gel filtration Xylella fastidiosa

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa Q9PBQ1
-
-
Xylella fastidiosa 9a5c Q9PBQ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Xylella fastidiosa 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Xylella fastidiosa 9a5c 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35900, SDS-PAGE Xylella fastidiosa

Synonyms

Synonyms Comment Organism
XF_2089
-
 Xylella fastidiosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Xylella fastidiosa

Expression

Organism Comment Expression
Xylella fastidiosa enzyme is overexpressed during Xylella fastidiosa biofilm formation, particularly in the early stages of its formation up