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Literature summary for 3.1.3.5 extracted from

  • Srinivasan, B.; Forouhar, F.; Shukla, A.; Sampangi, C.; Kulkarni, S.; Abashidze, M.; Seetharaman, J.; Lew, S.; Mao, L.; Acton, T.B.; Xiao, R.; Everett, J.K.; Montelione, G.T.; Tong, L.; Balaram, H.
    Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila (2014), FEBS J., 281, 1613-1628.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.5

Activating Compound

Activating Compound Comment Organism Structure
dGTP heterotrophic allosteric activator Legionella pneumophila
GDP heterotrophic allosteric activator Legionella pneumophila
GMP substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila
GTP heterotrophic allosteric activator Legionella pneumophila
Mg2+ preferred divalent cation Legionella pneumophila
Mn2+ may substitute for Mg2+ as divalent cation Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with phosphate and GMP, to 2.7 A nd 2.5 A resolution, respectively. Structures reveal an activator-binding site at the dimer interface Legionella pneumophila

Protein Variants

Protein Variants Comment Organism
R86A same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is compromised Legionella pneumophila
Y421S same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is compromised Legionella pneumophila
Y421S/R86A same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is abolished Legionella pneumophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2
-
 5'-GMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
18
-
 5'-IMP biphasic kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
142
-
 5'-dGMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila

Organism

Organism UniProt Comment Textmining
Legionella pneumophila Q5ZZB6
-
-
Legionella pneumophila DSM 7513 Q5ZZB6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-dGMP + H2O
-
 Legionella pneumophila deoxyguanosine + phosphate
-
 ?
5'-dGMP + H2O
-
 Legionella pneumophila DSM 7513 deoxyguanosine + phosphate
-
 ?
5'-dIMP + H2O
-
 Legionella pneumophila deoxyinosine + phosphate
-
 ?
5'-dIMP + H2O
-
 Legionella pneumophila DSM 7513 deoxyinosine + phosphate
-
 ?
5'-GMP + H2O preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila guanosine + phosphate
-
 ?
5'-GMP + H2O preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila DSM 7513 guanosine + phosphate
-
 ?
5'-IMP + H2O
-
 Legionella pneumophila inosine + phosphate
-
 ?
5'-IMP + H2O
-
 Legionella pneumophila DSM 7513 inosine + phosphate
-
 ?
additional information activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor Legionella pneumophila ?
-
 ?
additional information activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor Legionella pneumophila DSM 7513 ?
-
 ?

Synonyms

Synonyms Comment Organism
cN-II
-
 Legionella pneumophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 5'-IMP pH 8.0, temperature not specified in the publication Legionella pneumophila
12.4
-
 5'-GMP pH 8.0, temperature not specified in the publication Legionella pneumophila
20.7
-
 5'-dGMP pH 8.0, temperature not specified in the publication Legionella pneumophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 5'-IMP biphasic kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
0.15
-
 5'-dGMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
1.7
-
 5'-GMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila