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Literature summary for 3.1.3.5 extracted from
- Active and regulatory sites of cytosolic 5'-nucleotidase (2010), FEBS J., 277, 4863-4872.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-bisphosphoglycerate | - |
Bos taurus |  |
| ADP | - |
Bos taurus | |
| ATP | - |
Bos taurus | |
| diadenosine polyphosphate | - |
Bos taurus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged wild-type and mutant enzymes | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D396A | site-directed mutagenesis, the mutant is only activable by 2,3-bisphosphoglycerate | Bos taurus |
| F127E | site-directed mutagenesis | Bos taurus |
| F36R | site-directed mutagenesis, inactive mutant | Bos taurus |
| G319D | site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme | Bos taurus |
| H428D | site-directed mutagenesis | Bos taurus |
| I152D | site-directed mutagenesis | Bos taurus |
| K311A | site-directed mutagenesis, the mutant shows altered kinetics compare to the wild-type enzyme | Bos taurus |
| M436W | site-directed mutagenesis | Bos taurus |
| N154D | site-directed mutagenesis, the mutant shows a decrease in the value of K50 for Mg2+ compared to the wild-type enzyme | Bos taurus |
| R144E | site-directed mutagenesis | Bos taurus |
| T56V | site-directed mutagenesis | Bos taurus |
| Y115A | site-directed mutagenesis, the mutant behaves similar to the wild-type enzyme | Bos taurus |
| Y55G | site-directed mutagenesis | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant K311A | Bos taurus | |
| 0.05 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant G319D | Bos taurus | |
| 0.06 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant mutant Y115A | Bos taurus | |
| 0.1 | - |
5'-IMP | pH not specified in the publication, temperature not specified in the publication, recombinant wild-type enzyme and mutant D396A | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-IMP + H2O | Bos taurus | - |
inosine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | O46411 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-IMP + H2O | - |
Bos taurus | inosine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | the active tetramer is formed by interaction between two identical dimers via interface B: this interface contains 28 aminoacid residues, of which 8 make hydrogen bonds | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cN-II | - |
Bos taurus |
| cytosolic 5'-nucleotidase | - |
Bos taurus |
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