Cloned (Comment) | Organism |
---|---|
catalytically active recombinant soluble glutathione-S transferase/ecto-5'-NT fusion protein is expressed in insect cells using the baculovirus system | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
ADP | - |
Rattus norvegicus | |
alpha,beta-methylene-ADP | - |
Homo sapiens | |
alpha,beta-methylene-ADP | - |
Rattus norvegicus | |
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent | Homo sapiens | |
Mg2+ | dependent | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
agarose-coupled GSH affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
melanoma cell | - |
Homo sapiens | - |
melanoma cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | - |
Homo sapiens | adenosine + phosphate | - |
? | |
5'-AMP + H2O | 100% activity | Rattus norvegicus | adenosine + phosphate | - |
? | |
5'-CMP + H2O | 36% dephosphorylation in relation to AMP | Rattus norvegicus | cytidine + phosphate | - |
? | |
5'-GMP + H2O | 57% dephosphorylation in relation to AMP | Rattus norvegicus | guanosine + phosphate | - |
? | |
5'-UMP + H2O | 51% dephosphorylation in relation to AMP | Rattus norvegicus | uridine + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CD73 | - |
Homo sapiens |
CD73 | - |
Rattus norvegicus |
ecto-5'-NT | - |
Homo sapiens |
ecto-5'-NT | - |
Rattus norvegicus |
ecto-5'-nucleotidase | - |
Homo sapiens |
ecto-5'-nucleotidase | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00051 | - |
alpha,beta-methylene-ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Homo sapiens | |
0.00087 | - |
alpha,beta-methylene-ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus | |
0.00091 | - |
ADP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus | |
0.0089 | - |
ATP | in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 | Rattus norvegicus |