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Literature summary for 3.1.3.5 extracted from

  • Zagursky, R.J.; Ooi, P.; Jones, K.F.; Fiske, M.J.; Smith, R.P.; Green, B.A.
    Identification of a Haemophilus influenzae 5'-nucleotidase protein: cloning of the nucA gene and immunogenicity and characterization of the NucA protein (2000), Infect. Immun., 68, 2525-2534.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine the enzyme is a potential subunit vaccine candidate against NTHi disease Haemophilus influenzae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, recombinant enzyme is localized in periplasm of Escherichia coli Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 3.0 mM, 75% inhibition of recombinant enzyme, 20% inhibition of native enzyme Haemophilus influenzae

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information recombinant enzyme is localized in periplasm of Escherichia coli Haemophilus influenzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 3.0 mM, maximal stimulation Haemophilus influenzae
Mn2+ 3.0 mM, stimulation Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
x * 63000, SDS-PAGE Haemophilus influenzae
66200
-
x * 66200, calculation from nucleotide sequence Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
NTHi strain P860295
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information enzyme is not a lipoprotein Haemophilus influenzae

Purification (Commentary)

Purification (Comment) Organism
-
Haemophilus influenzae

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Haemophilus influenzae
? x * 66200, calculation from nucleotide sequence Haemophilus influenzae