Literature summary for 3.1.3.48 extracted from

Ferrari, E.; Tinti, M.; Costa, S.; Corallino, S.; Nardozza, A.P.; Chatraryamontri, A.; Ceol, A.; Cesareni, G.; Castagnoli, L.
Identification of new substrates of the protein-tyrosine phosphatase PTP1B by Bayesian integration of proteome evidence (2011), J. Biol. Chem., 286, 4173-4185.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of PTP1B in HEK-293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of stable cell lines expressing a short hairpin RNA targeting the phosphatase transcript and reduction of PTP1B expression levels down to 20%. Upon PTP1B overexpression, phosphorylation of PLC-gamma1 and Gab1 sharply decreases when compared with controls where PLC-gamma1 and Gab1 phosphorylations	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gab1 tyrosine phosphate + H2O	-	Homo sapiens	Gab1 tyrosine + phosphate	-	?
additional information	substrate specificity analysis using a trapping mutant of the phosphatase, which is a point mutation that maintains specificity but does not allow detachment of the phosphate, overview. The enzyme is also active on 4-nitrophenyl phosphate, but is not active on GRB2	Homo sapiens	?	-	?
PLC-gamma1 tyrosine phosphate + H2O	-	Homo sapiens	PLC-gamma1 tyrosine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PTP1B	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	phosphatases interact only transiently with their substrates	Homo sapiens
physiological function	PTP1B-mediated dephosphorylation of Gab1 negatively affects its EGF-induced association with the phosphatase SHP2. The dissociation of this signaling complex is accompanied by a decrease of ERK MAP kinase phosphorylation and activation. PTP1B specifically interferes with SHP2 recruitment by Gab1 and with ERK1/2 activation	Homo sapiens

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Release 2023.1 (January 2023)