Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged enzyme, hanging drop vapor diffusion method, mixing of 0.0018 ml of protein solution, containing 18 mg/ml protein in 20 mM HEPES-NaOH, pH 7.0, 0.2 M NaCl, 5 mM MgCl2, 20 mM DTT, and 5% glycerol, with an equal volume of reservoir solution containing 0.1 M bis-Tris, pH 6.0, and 2.4 M NaCl, 18°C, 3 days, X-ray diffraction structure determination and analysis at 2.4 A resolution | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | ED-Eya2 is bifunctional acting as protein tyrosine phosphatase and as transcription factor. The transcriptional activity of Eya proteins is regulated by a dephosphorylating activity within its Eya domain, structure-function analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00167 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ED-Eya2 is bifunctional acting as protein tyrosine phosphatase and as transcription factor. The transcriptional activity of Eya proteins is regulated by a dephosphorylating activity within its Eya domain, structure-function analysis, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | ED-Eya2 shows a helix-bundle motif that elongated along the back of the catalytic site | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ED-Eya2 | - |
Homo sapiens |
More | ED-Eya2 belongs to the haloacid dehalogenase family | Homo sapiens |