Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with monophosphorylated and dually phosphorylated Erk2 peptides, sitting drop vapour diffusion method, in 0.2 M ammonium tartrate, pH 6.6, and 20% (w/v) PEG 3350 or and 1.0 M lithium chloride, 0.1 M citrate, pH 5.0, and 20% (w/v) PEG 6000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C270A | inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) | Homo sapiens |
C270S | inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) | Homo sapiens |
C270S/T106D | inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) | Homo sapiens |
D236A | substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 420fold reduced activity compared to the wild type enzyme | Homo sapiens |
D236A/C270A/Q314A | inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) | Homo sapiens |
D236A/C270S/Q314A | inactive, substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339) | Homo sapiens |
D236A/Q314A | substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 1255fold reduced activity compared to the wild type enzyme | Homo sapiens |
Q314A | substrate-trapping mutant of the HePTP catalytic domain (HePTP residues 44-339), shows 13fold reduced activity compared to the wild type enzyme | Homo sapiens |
T106D | KIM-PTP specific substrate-trapping mutant, the introduced aspartate side chain facilitates the coordination of the bound peptides, thereby stabilizing the active dephosphorylation complex | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
4-nitrophenyl phosphate | mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
1.31 | - |
4-nitrophenyl phosphate | mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
2.11 | - |
4-nitrophenyl phosphate | mutant enzyme Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
3.26 | - |
4-nitrophenyl phosphate | wild type enzyme, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35236 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Superdex 75 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hematopoietic cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
LDEpYVATR + H2O | - |
Homo sapiens | LDEYVATR + phosphate | - |
? | |
phosphorylated ERK1 + H2O | - |
Homo sapiens | ERK1 + phosphate | - |
? | |
phosphorylated ERK2 + H2O | HePTP binds the phosphorylated tyrosine of the Erk2 peptide (pY185), Erk2 residue T183, which is phosphorylated in maximally activated Erk2, is not essential for substrate recognition and binding by HePTP | Homo sapiens | ERK2 + phosphate | - |
? | |
phosphorylated p38 mitogen-activated protein kinase + H2O | - |
Homo sapiens | p38 mitogen-activated protein kinase + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hematopoietic tyrosine phosphatase | - |
Homo sapiens |
HEPTP | HePTP negatively regulates T cell activation and proliferation | Homo sapiens |
KIM-PTP | - |
Homo sapiens |
kinase interaction motif phosphatase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
4-nitrophenyl phosphate | mutant enzyme D236A/Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
0.03 | - |
4-nitrophenyl phosphate | mutant enzyme D236A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
0.93 | - |
4-nitrophenyl phosphate | mutant enzyme Q314A, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens | |
12.55 | - |
4-nitrophenyl phosphate | wild type enzyme, at 30°C, in 0.15 M Bis-Tris, pH 6.0 | Homo sapiens |