Literature summary for 3.1.3.4 extracted from

Choi, H.S.; Su, W.M.; Han, G.S.; Plote, D.; Xu, Z.; Carman, G.M.
Pho85p-Pho80p phosphorylation of yeast Pah1p phosphatidate phosphatase regulates its activity, location, abundance, and function in lipid metabolism (2012), J. Biol. Chem., 287, 11290-11301.

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Protein Variants

Protein Variants	Comment	Organism
S110A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
S114A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
S168A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
S602A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
S744A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
S748A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae
T723A	phosphorylation site mutant, mutation diminishes inhibitory effects of phosphorylation by Pho85p-Pho80p	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P32567	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation at sites Ser110, Ser114, Ser168, Ser602, Thr723, Ser744, and Ser748 is managed by protein kinase-cyclin complex Pho85p-Pho80p. The phosphorylation of recombinant isoform Pah1p is time- and dose-dependent and dependent on the concentrations of ATP and Pah1p. Phosphorylation reduces the catalytic efficiency 6fold and reduces 3fold its interaction with liposomes. Alanine mutations of the seven sites ablates the inhibitory effect of phosphorylation. Loss of Pho85p-Pho80p phosphorylation reduces Pah1p abundance. Lack of Nem1p-Spo7p, the phosphatase complex that dephosphorylates Pah1p at the nuclear/endoplasmic reticulum membrane, stabilizes Pah1p abundance	Saccharomyces cerevisiae

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
Pah1p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)