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Literature summary for 3.1.3.4 extracted from

  • Nakamura, Y.; Koizumi, R.; Shui, G.; Shimojima, M.; Wenk, M.R.; Ito, T.; Ohta, H.
    Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation (2009), Proc. Natl. Acad. Sci. USA, 106, 20978-20983.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
full-length coding sequence of PAH1 and PAH2 cloned into the pDO105 vector at NotI/MluI sites for PAH1 and NotI/PstI sites for PAH2. Vector constructs introduced into a Saccharomyces cerevisiae DELTAdpp1DELTAlpp1DELTApah1 mutant. Transgenic pah1pah2 plants that harbor either 35S::PAH1-GFP or 35S::PAH2-GFP transgenes Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble PAH1 and PAH2 Arabidopsis thaliana
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-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ depends on Saccharomyces cerevisiae
Mg2+ depends on Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
 PAH1 and PAH2, calculated from sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
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Saccharomyces cerevisiae
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-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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 Arabidopsis thaliana
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rosette leaf
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 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidic acid + H2O both PAH1 and PAH2 have two domains, the amino-terminal lipin and carboxy-terminal lipin domains. PAH1 and PAH2 may supply diacylglycerol as a substrate of galactolipid synthesis, and phosphatidic acid hydrolyzed by PAH1 and PAH2 may be derived from phosphatidylcholine and phosphatidylethanolamine Arabidopsis thaliana 1,2-diacyl-sn-glycerol + phosphate
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 ?

Synonyms

Synonyms Comment Organism
lipin
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 Saccharomyces cerevisiae
lipin
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 Arabidopsis thaliana
pah1
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 Arabidopsis thaliana
Pah1p
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 Saccharomyces cerevisiae
PAH2
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 Arabidopsis thaliana
PAP
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 Saccharomyces cerevisiae
PAP
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 Arabidopsis thaliana
phosphatidate phosphatase
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 Saccharomyces cerevisiae
phosphatidate phosphatase
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 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana homozygous T-DNA-tagged mutants of PAH1 and PAH2 do not express their respective full-length mRNAs down

General Information

General Information Comment Organism
malfunction double mutant pah1pah2 plants have decreased phosphatidic acid hydrolysis, thus affecting the eukaryotic pathway of galactolipid synthesis. Upon phosphate starvation, pah1pah2 plants are severely impaired in growth and membrane lipid remodeling. PAP activity in the supernatant fraction of pah1pah2 mutant leaves is decreased by approximately 40% as compared to that in wild-type leaves. Defect in PAP activity in vivo in rosette leaves of pah1pah2 mutants. Relative amount of phosphatidic acid increases to 1.61fold in pah1pah2 double mutants as compared to the wild-type. 26% increase in phosphatidic acid levels in pah1pah2 plants as compared to wild-type plants. The transgenic plants (35S::PAH1-GFP, pah1pah2 and 35S::PAH2-GFP, pah1pah2) recover the phenotype observed in pah1pah2 mutant. Endoplasmic reticulum-localized eukaryotic pathway of membrane lipid metabolism is compromised in pah1pah2 double mutants Arabidopsis thaliana
malfunction yeast DELTAdpp1DELTAlpp1DELTApah1 mutant is complemented by Arabidopsis phosphatidate phosphatases PAH1 and PAH2 in vivo Saccharomyces cerevisiae
physiological function PAH1 and PAH2 are the phosphatidate phosphatase responsible for the eukaryotic pathway of galactolipid synthesis. Membrane lipid remodeling mediated by these two enzymes is an essential adaptation mechanism to cope with phosphate starvation. Complements yeast DELTAdpp1DELTAlpp1DELTApah1 in vivo Arabidopsis thaliana