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Literature summary for 3.1.3.4 extracted from

  • Havriluk, T.; Lozy, F.; Siniossoglou, S.; Carman, G.
    Colorimetric determination of pure Mg2+-dependent phosphatidate phosphatase activity (2008), Anal. Biochem., 373, 392-394.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the PAP1 colorimetric assay with pure enzyme and a water-soluble phosphatidate substrate is applicability to a 96-well format, which may facilitate a large-scale screen of PAP1 inhibitors (or activators) Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
DMSO addition to the reaction mixture results in a dose-dependent inhibition of PAP1 activity, 25% loss of PAP1 activity at a 1% concentration Saccharomyces cerevisiae
Phenylglyoxal is an arginine reactive compound, inhibits PAP1 activity in dose-dependent manner Saccharomyces cerevisiae
propranolol interacts with the Mg2+-binding site of the enzyme, inhibits PAP1 activity in dose-dependent manner Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ colorimetric assay Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2
-
pure enzyme, radioactive assay Saccharomyces cerevisiae
5.6
-
pure enzyme, colorimetric assay Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dioctanoyl phosphatidic acid + H2O PAP1 activity is linear with respect to the substrate at concentrations between 0.05-0.8 mM Saccharomyces cerevisiae 1,2-dioctanoyl-sn-glycerol + phosphate
-
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Synonyms

Synonyms Comment Organism
3-sn-phosphatidate phosphohydrolase
-
Saccharomyces cerevisiae
Mg2+-dependent PA1 phosphatase
-
Saccharomyces cerevisiae
Mg2+-dependent phosphatidate phosphatase
-
Saccharomyces cerevisiae
pah1
-
Saccharomyces cerevisiae
PAP1
-
Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.2
-
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate Saccharomyces cerevisiae propranolol
1.3
-
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate Saccharomyces cerevisiae Phenylglyoxal