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Literature summary for 3.1.3.4 extracted from
- Vacuole membrane topography of the DPP1-encoded diacylglycerol pyrophosphate phosphatase catalytic site from Saccharomyces cerevisiae (2004), J. Biol. Chem., 279, 5338-5345.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase, stress condition of zinc depletion induces DPP1 expression | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc depletion increases the enzyme activity in vivo, stress condition of zinc depletion induces DPP1 expression | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| vacuolar membrane | associated, the enzyme contains six putative transmembrane domains, the catalytic site is oriented to the cytosolic face of the vacuole membrane | Saccharomyces cerevisiae | 5774 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerol diphosphate + H2O | Saccharomyces cerevisiae | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | phosphatidate + phosphate | - |
? | |
| diacylglycerol diphosphate + H2O | Saccharomyces cerevisiae W303-1A | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | phosphatidate + phosphate | - |
? | |
| phosphatidate + H2O | Saccharomyces cerevisiae | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | diacyl-sn-glycerol + phosphate | - |
? | |
| phosphatidate + H2O | Saccharomyces cerevisiae W303-1A | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | diacyl-sn-glycerol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene DPP1 | - |
| Saccharomyces cerevisiae W303-1A | - |
gene DPP1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| preparation of vacuoles | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate | catalytic site structure within the transverse plane of the vacuole membrane, overview | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
recombinant vacuole membranes of intact vacuoles from overexpressing transgenic mutant DTY1 | Saccharomyces cerevisiae |
| 7 | - |
recombinant vacuole membranes of Triton X-100-ruptured vacuoles from overexpressing transgenic mutant DTY1 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerol diphosphate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | Saccharomyces cerevisiae | phosphatidate + phosphate | - |
? | |
| diacylglycerol diphosphate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol | Saccharomyces cerevisiae | phosphatidate + phosphate | - |
? | |
| diacylglycerol diphosphate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | Saccharomyces cerevisiae W303-1A | phosphatidate + phosphate | - |
? | |
| diacylglycerol diphosphate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol | Saccharomyces cerevisiae W303-1A | phosphatidate + phosphate | - |
? | |
| phosphatidate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | Saccharomyces cerevisiae | diacyl-sn-glycerol + phosphate | - |
? | |
| phosphatidate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol | Saccharomyces cerevisiae | diacyl-sn-glycerol + phosphate | - |
? | |
| phosphatidate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview | Saccharomyces cerevisiae W303-1A | diacyl-sn-glycerol + phosphate | - |
? | |
| phosphatidate + H2O | the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol | Saccharomyces cerevisiae W303-1A | diacyl-sn-glycerol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme has six putative transmembrane domains and a hydrophilic region that contains a phosphatase motif required for its catalytic activity, membrane topology, overview | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DGPP phosphatase | - |
Saccharomyces cerevisiae |
| diacylglycerol diphosphate phosphatase | - |
Saccharomyces cerevisiae |
| diacylglycerol pyrophosphate phosphatase | - |
Saccharomyces cerevisiae |
| DPP1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Saccharomyces cerevisiae |
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