Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | 70% inhibition of isozyme PAP-1; 70% inhibition of isozyme PAP-1, 30% inhibition of isozyme PAP-2 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphate | pH 7.5, 37°C, crude enzyme preparation | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | isozyme PAP-1, dependent on | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | phosphatidic acid phosphohydrolase, PAP, catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol, the second messenger responsible for activation of protein kinase C | ? | - |
? | |
additional information | Rattus norvegicus | phosphatidic acid phosphohydrolase, PAP, catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol, the second messenger responsible for activation of protein kinase C | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes PAP-1 and PAP-2 | - |
Rattus norvegicus | - |
isozyme PAP-2 | - |
Rattus norvegicus | - |
isozymes PAP-1 and PAP-2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | approximately fourfold higher in ventricular myocardium than in atrial tissue | Homo sapiens | - |
heart | ventricular and atrial tissue, isozyme PAP-2 | Rattus norvegicus | - |
heart | ventricular and atrial tissue, isozymes PAP-1 and PAP-2 | Rattus norvegicus | - |
myocardium | - |
Homo sapiens | - |
myocardium | isozyme PAP-2 | Rattus norvegicus | - |
myocardium | isozymes PAP-1 and PAP-2 | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a HPLC-fluorescence detection method, overview | Homo sapiens |
additional information | - |
development of a HPLC-fluorescence detection method, overview | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphate + H2O | fluorescent substrate deirvative | Homo sapiens | 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycerol + phosphate | - |
? | |
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphate + H2O | fluorescent substrate deirvative | Rattus norvegicus | 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycerol + phosphate | - |
? | |
2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphate + H2O | fluorescent substrate derivative | Rattus norvegicus | 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycerol + phosphate | - |
? | |
additional information | phosphatidic acid phosphohydrolase, PAP, catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol, the second messenger responsible for activation of protein kinase C | Homo sapiens | ? | - |
? | |
additional information | phosphatidic acid phosphohydrolase, PAP, catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol, the second messenger responsible for activation of protein kinase C | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP-1 | - |
Homo sapiens |
PAP-1 | - |
Rattus norvegicus |
PAP-2 | - |
Homo sapiens |
PAP-2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |