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Literature summary for 3.1.3.4 extracted from
- The Saccharomyces cerevisiae lipin homologue is a Mg2+-dependent phosphatidate phosphatase enzyme (2006), J. Biol. Chem., 281, 9210-9218.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-overexpression of lipin with murine LPP-1 in Escherichia coli strain BL21(DE3) as His-tagged enzymes | Homo sapiens |
| gene PAH1, overexpression of His-tagged wild-type and mutant isozyme PAH1 in Escherichia coli strain BL21(DE3), co-expression with human lipin, subcloning in Escherichia coli strain DH5alpha | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a PAH1 mutant with reduced enzyme activity, the mutant grows more slowly and is temperature-sensitive at 37°C, mutant cells show increased phosphatidate levels compared to the wild-type cells, overview | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, recombinant wild-type and mutant PAH1, overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| membrane | associated | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Homo sapiens |  |
| Mg2+ | dependent on | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidic acid + H2O | Homo sapiens | - |
monoacylglycerol + phosphate | - |
? | |
| lysophosphatidic acid + H2O | Saccharomyces cerevisiae | - |
monoacylglycerol + phosphate | - |
? | |
| phosphatidic acid + H2O | Homo sapiens | - |
1,2-diacylglycerol + phosphate | - |
? | |
| phosphatidic acid + H2O | Saccharomyces cerevisiae | - |
diacylglycerol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Saccharomyces cerevisiae | - |
several strains, isozyme PAH1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged lipin from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
| recombinant His-tagged wild-type and mutant isozyme PAH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant wild-type and mutant PAH1, assay in presence of Triton X-100 at 2 mM | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidic acid + H2O | - |
Homo sapiens | monoacylglycerol + phosphate | - |
? | |
| lysophosphatidic acid + H2O | - |
Saccharomyces cerevisiae | monoacylglycerol + phosphate | - |
? | |
| additional information | the enzyme is homologous to mammalian lipin | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the enzyme is homologous to murine lipid phosphate phosphatase isozyme LPP-1 | Homo sapiens | ? | - |
? | |
| phosphatidic acid + H2O | - |
Homo sapiens | 1,2-diacylglycerol + phosphate | - |
? | |
| phosphatidic acid + H2O | - |
Saccharomyces cerevisiae | diacylglycerol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipin | - |
Homo sapiens |
| PA phosphatase | - |
Homo sapiens |
| PA phosphatase | - |
Saccharomyces cerevisiae |
| PAH | - |
Homo sapiens |
| PAH | - |
Saccharomyces cerevisiae |
| SMP2 | formerly | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at, with substrate lysophosphatidic acid | Saccharomyces cerevisiae |
| 7.5 | - |
assay at, with substrate phosphatidic acid | Saccharomyces cerevisiae |
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