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Literature summary for 3.1.3.4 extracted from
- Characterization of the pulmonary N-ethylmaleimide-insensitive phosphatidate phosphohydrolase (2000), Exp. Lung Res., 26, 361-381.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 60% inhibition of PAP-2 | Rattus norvegicus |  |
| ceramide 1-phosphate | - |
Rattus norvegicus | |
| chlorpromazine | 55% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
| diacylglycerol | - |
Rattus norvegicus | |
| F- | PAP-2 | Rattus norvegicus | |
| lysophosphatidic acid | - |
Rattus norvegicus | |
| Mg2+ | 15 mM MgCl2, 10-20% inhibition of PAP-2 | Rattus norvegicus | |
| monoacylglycerol | - |
Rattus norvegicus | |
| NEM | 2 mM, complete inhibition of PAP-1. NEM, up to 8 mM has little effect on PAP-2 | Rattus norvegicus | |
| spermidine trihydrochloride | 7% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
| spermine | 55% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
| sphingomyelin | 72% inhibition at equimolar concentration with pure phosphatidic acid. No effect with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine | Rattus norvegicus | |
| sphingosine | 93% inhibition at equimolar concentration with pure phosphatidic acid. 29% inhibition with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine | Rattus norvegicus | |
| sphingosine 1-phosphate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Rattus norvegicus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | PAP-1 is dependent on | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
2 different phosphatidate phosphatidases: PAP-1 and PAP-2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| alveolar cell type II | PAP-2 | Rattus norvegicus | - |
| lung | PAP-2 | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidic acid + H2O | - |
Rattus norvegicus | ? | - |
? | |
| additional information | key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis | Rattus norvegicus | ? | - |
? | |
| phosphatidic acid + H2O | phosphatidic acid pure or in mixed micelles with phosphatidylcholine | Rattus norvegicus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
| sphingosine 1-phosphate + H2O | poor substrate | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP-1 | - |
Rattus norvegicus |
| PAP-2 | - |
Rattus norvegicus |
| PAPase | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
activity of PAP-2 is not seriously affected after 20 min | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
phosphatidic acid mixed with phosphatidylcholine, PAP-2 | Rattus norvegicus |
| 6.5 | - |
pure phosphatidic acid, PAP-2 | Rattus norvegicus |
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