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Literature summary for 3.1.3.4 extracted from

  • Fleming, I.N.; Yeaman, S.J.
    Purification and characterization of N-ethylmaleimide-insensitive phosphatidic acid phosphohydrolase (PAP2) from rat liver (1995), Biochem. J., 308, 983-989.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.4

Inhibitors

Inhibitors Comment Organism Structure
1,2-diacylglycerol
-
 Rattus norvegicus
amphiphilic amines
-
 Rattus norvegicus
F- enzyme form PAP2 Rattus norvegicus
Mn2+
-
 Rattus norvegicus
NEM no inhibition Rattus norvegicus
propanolol partial Rattus norvegicus
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 phosphatidic acid enzyme form PAP2B Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, enzyme form PAP2B, SDS-PAGE Rattus norvegicus
265000
-
 native PAGE Rattus norvegicus
290000 300000 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two forms of PAP2: PAP2A and PAP2B Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate + H2O
-
 Rattus norvegicus 1-stearoyl-2-arachidonoyl-sn-glycerol + phosphate
-
 ?
didecanoyl phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-didecanoyl-sn-glycerol + phosphate
-
 ?
dihexanoyl phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-dihexanoyl-sn-glycerol + phosphate
-
 ?
dimyristoyl phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-dimyristoyl-sn-glycerol + phosphate
-
 ?
dioleoyl phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-dioleoyl-sn-glycerol + phosphate
-
 ?
distearoyl phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-distearoyl-sn-glycerol + phosphate
-
 ?
phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-diacyl-sn-glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, enzyme form PAP2B, SDS-PAGE Rattus norvegicus