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Literature summary for 3.1.3.4 extracted from

  • Hosaka, K.; Yamashita, S.
    Partial purification and properties of phosphatidate phosphatase in Saccharomyces cerevisiae (1984), Biochim. Biophys. Acta, 796, 102-109.
    View publication on PubMed
Search for more literature for 3.1.3.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 phosphatidic acid
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mn2+ slight stimulation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.37
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, stable for at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-acyl-sn-glycerol 3-phosphate + H2O less than 15% of the activity with phosphatidic acid Saccharomyces cerevisiae 1-acyl-sn-glycerol + phosphate
-
 ?
phosphatidic acid + H2O highly specific for Saccharomyces cerevisiae 1,2-diacyl-sn-glycerol + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: 83% of maximal activity, pH 9.0: about 36% of maximal activity Saccharomyces cerevisiae