Application | Comment | Organism |
---|---|---|
molecular biology | translocatable phosphatase: method is based on the rapamycin-induced heterodimerization of FRB (fragment of mammalian target of rapamycin that binds FKB12) and FKB12 (FK506 binding protein 12). A mutant version of 5-ptase with a defective membrane targeting domain (CAAX box) is fused to FKB12 and tagged with monomeric red fluorescent protein, whereas its binding partner FRB (fused to CFP) is tethered to the plasma membrane through palmitoylation. In the absence of rapamycin, 5-ptase resides in the cytosol and leaves D-myo-phosphatidylinositol 4,5-bisphosphate levels at the plasma membrane unaltered. Upon addition of 100 nM rapamycin, FKB12 and FRB undergo heterodimerization and the 5-ptase is recruited to the plasma membrane | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | using the translocatable 5-phosphatase method, it is shown that upon addition of rapamycin, which causes a rapid depletion of D-myo-phosphatidylinositol 4,5-bisphosphate, Cx43 gap junctional communication is closed | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-phosphatidylinositol 4,5-bisphosphate | - |
Homo sapiens | D-myo-phosphatidylinositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-ptase | - |
Homo sapiens |
PIP2 5-phosphatase | - |
Homo sapiens |
type IV phosphoinositide 5-phoshatase | - |
Homo sapiens |