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Literature summary for 3.1.3.3 extracted from

  • Jung, T.Y.; Kim, Y.S.; Oh, B.H.; Woo, E.
    Identification of a novel ligand binding site in phosphoserine phosphatase from the hyperthermophilic archaeon Thermococcus onnurineus (2013), Proteins, 81, 819-829.
    View publication on PubMed
Search for more literature for 3.1.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus onnurineus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method at 18 °C, crystal structure of the enzyme at 1.8 A resolution Thermococcus onnurineus

Inhibitors

Inhibitors Comment Organism Structure
1-(3-sulfopropyl)pyridin-1-ium
-
 Thermococcus onnurineus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 O-phospho-L-serine pH 6.5, 65°C Thermococcus onnurineus
0.088
-
 O-phospho-L-serine pH 6.5, 37°C Thermococcus onnurineus
0.1
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 20 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
0.14
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 100 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
0.181
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 200 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + H2O Thermococcus onnurineus the enzyme catalyzes the final and irreversible step of L-serine synthesis L-serine + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus B6YX36
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-serine + H2O
-
 Thermococcus onnurineus L-serine + phosphate
-
 ir
O-phospho-L-serine + H2O the enzyme catalyzes the final and irreversible step of L-serine synthesis Thermococcus onnurineus L-serine + phosphate
-
 ir

Synonyms

Synonyms Comment Organism
PSP
-
 Thermococcus onnurineus
TON_1161 locus name Thermococcus onnurineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermococcus onnurineus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 75 60°C: about 50% of maximal activity, 75°C: about 75% of maximal activity Thermococcus onnurineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0073
-
 O-phospho-L-serine pH 6.5, 37°C Thermococcus onnurineus
0.143
-
 O-phospho-L-serine pH 6.5, 65°C Thermococcus onnurineus
0.168
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 20 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
0.179
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 100 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
0.182
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 200 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Thermococcus onnurineus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0824
-
 O-phospho-L-serine pH 6.5, 37°C Thermococcus onnurineus
1.01
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 200 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
1.32
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 100 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
1.69
-
 O-phospho-L-serine pH 6.5, 65°C, in presence of 20 mM 3-(1-pyridinio)-1-propanesulfonate Thermococcus onnurineus
1.81
-
 O-phospho-L-serine pH 6.5, 65°C Thermococcus onnurineus