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Literature summary for 3.1.3.3 extracted from

  • Tribble, G.D.; Mao, S.; James, C.E.; Lamont, R.J.
    A Porphyromonas gingivalis haloacid dehalogenase family phosphatase interacts with human phosphoproteins and is important for invasion (2006), Proc. Natl. Acad. Sci. USA, 103, 11027-11032.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DH5alpha Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
D198N 9% activity of the wild type enzyme Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Porphyromonas gingivalis
Na3VO4
-
Porphyromonas gingivalis
NaF
-
Porphyromonas gingivalis
okadaic acid
-
Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
L-phosphotyrosine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis
0.117
-
L-phosphothreonine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis
2.041
-
L-phosphoserine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
BioLogic DuoFlow chromatography system loaded with an IMAC column Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerinaldehyde-3-phosphate-dehydrogenase + H2O
-
Porphyromonas gingivalis ?
-
?
heat-shock protein 90 + H2O
-
Porphyromonas gingivalis ?
-
?
L-phosphoserine + H2O
-
Porphyromonas gingivalis L-Ser + phosphate
-
?
L-phosphothreonine + H2O
-
Porphyromonas gingivalis L-Thr + phosphate
-
?
O-phospho-L-tyrosine + H2O
-
Porphyromonas gingivalis L-Tyr + phosphate
-
?

Synonyms

Synonyms Comment Organism
SerB PG0653
-
Porphyromonas gingivalis
SerB653 is a HAD-family phosphatase Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00003
-
L-phosphotyrosine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis
0.0004
-
L-phosphothreonine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis
1.508
-
L-phosphoserine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis
2.94
-
L-phosphoserine at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.005
-
IC50: above 0.005 mM, at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis okadaic acid
1.3
-
at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis Na3VO4
4.4
-
at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis EDTA
5.9
-
at 30°C, in 50 mM Tris (pH 7.5), with 5 mM MgCl2 Porphyromonas gingivalis NaF