Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl phosphate | competitive | Nicotiana tabacum | |
ATP | 1 mM, 29% residual activity, competitive | Nicotiana tabacum | |
EDTA | 1 mM, 62% residual activity | Nicotiana tabacum | |
fluoride | 1 mM, 56% residual activity | Nicotiana tabacum | |
molybdate | 1 mM, 5% residual activity | Nicotiana tabacum | |
additional information | not inhibitory: levamisole and tartrate | Nicotiana tabacum | |
phosphate | 1 mM, 20% residual activity, competitive | Nicotiana tabacum | |
pyridoxal | 1 mM, 20% residual activity, competitive | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
pyridoxal 5'-phosphate | pH 5.5, 37°C | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 179% of initial activity | Nicotiana tabacum | |
Mg2+ | most effective dimetal ion for catalysis under saturating substrate concentrations, 1 mM, 215% of initial activity | Nicotiana tabacum | |
Mn2+ | 1 mM, 154% of initial activity | Nicotiana tabacum | |
additional information | a divalent metal ion is required for activity | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24900 | - |
2 * 24900, SDS-PAGE | Nicotiana tabacum |
50000 | - |
gel filtration | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1998 | - |
pH 5.5, 37°C | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Nicotiana tabacum | 4-nitrophenol + phosphate | about 158% of the activity with pyridoxal 5'-phosphate | ? | |
diphosphate + H2O | - |
Nicotiana tabacum | 2 phosphate | about 156% of the activity with pyridoxal 5'-phosphate | ? | |
pyridoxal 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxal + phosphate | - |
? | |
pyridoxamine 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxamine + phosphate | about 50% of the activity with pyridoxal 5'-phosphate | ? | |
pyridoxine 5'-phosphate + H2O | - |
Nicotiana tabacum | pyridoxine + phosphate | about 50% of the activity with pyridoxal 5'-phosphate | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24900, SDS-PAGE | Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Nicotiana tabacum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
citric acid-sodium citrate buffer, pH 5.5, fully stable at least for 120 min | Nicotiana tabacum |
50 | - |
half-life around 50 min | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Nicotiana tabacum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 37°C for 2 h, more than 80% of total activities | Nicotiana tabacum |