Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-tagged fusion protein | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
AtTLP18.3 proteins are crystallized by the hanging drop vapor diffusion method at 23°C. X-ray crystallography reveal the folding of AtTLP18.3 as a three-layer sandwich with 3 alpha-helices in the upper layer, 4 beta-sheets in the middle layer, and 2 alpha-helices in the lower layer, which resembles a Rossmann fold | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.89 | - |
RRA(pT)VA | synthetic phosphorylated oligopeptide, pH 4, 37°C | Arabidopsis thaliana | |
12.49 | - |
Ac-(pT)AILER | synthetic phosphorylated oligopeptide, pH 4, 37°C | Arabidopsis thaliana | |
12.56 | - |
KR(pT)IRR | synthetic phosphorylated oligopeptide, pH 4, 37°C | Arabidopsis thaliana | |
13.88 | - |
Ac-RK(pS)AGKPKN | synthetic phosphorylated oligopeptide, pH 4, 37°C | Arabidopsis thaliana | |
16.57 | - |
Ac-(pT)IALGK | synthetic phosphorylated oligopeptide, pH 4, 37°C | Arabidopsis thaliana | |
42.56 | - |
4-nitrophenyl phosphate | pH 4, 37°C | Arabidopsis thaliana | |
47.51 | - |
O-phospho-L-serine | pH 4, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activity depends on divalent cations | Arabidopsis thaliana | |
Fe2+ | activity depends on divalent cations | Arabidopsis thaliana | |
Mg2+ | activity depends on divalent cations | Arabidopsis thaliana | |
Mn2+ | activity depends on divalent cations | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9ZVL6 | - |
- |
Purification (Comment) | Organism |
---|---|
using a GSTrap FF column | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
6,8 difluoro-4-methylumbelliferyl phosphate + H2O | - |
Arabidopsis thaliana | 6,8 difluoro-4-methylumbelliferol + phosphate | - |
? | |
Ac-(pT)AILER + H2O | synthetic phosphorylated oligopeptide | Arabidopsis thaliana | ? | - |
? | |
Ac-(pT)IALGK + H2O | synthetic phosphorylated oligopeptide | Arabidopsis thaliana | ? | - |
? | |
Ac-RK(pS)AGKPKN + H2O | synthetic phosphorylated oligopeptide | Arabidopsis thaliana | ? | - |
? | |
KR(pT)IRR + H2O | synthetic phosphorylated oligopeptide | Arabidopsis thaliana | ? | - |
? | |
O-phospho-L-serine + H2O | - |
Arabidopsis thaliana | L-serine + phosphate | - |
? | |
RRA(pT)VA + H2O | synthetic phosphorylated oligopeptide | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase | - |
Arabidopsis thaliana |
AtTLP18.3 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | highest activity | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | highest activity | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | Northern blot analysis reveal AtTLP18.3 with a diurnally fluctuating expression pattern in wild-type plants. The transcripts show 2 high expression peaks, at 8 and 16 h after lights are 14 turned on | up |