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Literature summary for 3.1.3.2 extracted from

  • Crowe, E.; Valladares, R.; Vu, C.; Kuznetsova, E.; Gonzalez, C.F.
    Determination of Francisella tularensis AcpB acid phosphatase substrate preferences (2010), J. Mol. Microbiol. Biotechnol., 19, 198-203.
    View publication on PubMed
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Francisella tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 Thiamine monophosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg Francisella tularensis
0.19
-
 thiamine diphosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg Francisella tularensis
0.47
-
 phosphocholine pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg Francisella tularensis
3.11
-
 4-nitrophenyl phosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg Francisella tularensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ improves activity Francisella tularensis
Fe2+ improves activity Francisella tularensis
Mg2+ improves activity Francisella tularensis
Mn2+ improves activity Francisella tularensis
Zn2+ improves activity Francisella tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Francisella tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Francisella tularensis 4-nitrophenol + phosphate
-
 ?
phosphocholine + H2O
-
 Francisella tularensis choline + phosphate
-
 ?
thiamine diphosphate + 2 H2O
-
 Francisella tularensis thiamine + 2 phosphate
-
 ?
thiamine monophosphate + H2O
-
 Francisella tularensis thiamine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
AcpB
-
 Francisella tularensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 thiamine diphosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 1.38 micromol/min/mg Francisella tularensis
4.48
-
 4-nitrophenyl phosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 11.84 micromol/min/mg Francisella tularensis
4.85
-
 Thiamine monophosphate pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 13.3 micromol/min/mg Francisella tularensis
33.24
-
 phosphocholine pH 37°C, pH 5.6, 1 mM MgCl2, Vmax: 89.58 micromol/min/mg Francisella tularensis

pH Range

pH Minimum pH Maximum Comment Organism
5.2 5.6
-
 Francisella tularensis