Cloned (Comment) | Organism |
---|---|
- |
Euphorbia characias |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
molybdate | inhibits enzymatic activity | Euphorbia characias | |
vanadate | inhibits enzymatic activity | Euphorbia characias | |
Zn2+ | inhibits enzymatic activity | Euphorbia characias |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
4-nitrophenyl phosphate | pH 5.75, 25°C | Euphorbia characias |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit | Euphorbia characias | |
Zn | ELPAP contains 1 mol of Fe and 1 mole of Zn per mol of subunit | Euphorbia characias |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
SDS-PAGE | Euphorbia characias |
130000 | - |
native protein, gel filtration | Euphorbia characias |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euphorbia characias | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, DEAE-cellulose chromatography and gel filtration chromatography | Euphorbia characias |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-bisphosphoglycerate + H2O | 85% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | ? | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Euphorbia characias | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | AMP + phosphate | - |
? | |
AMP + H2O | 16% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | adenosine + phosphate | - |
? | |
ATP + H2O | 100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | ADP + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | 25% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 104% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | D-glucose + phosphate | - |
? | |
GMP + H2O | 66% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | guanosine + phosphate | - |
? | |
inositol 1-phosphate + H2O | 60% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | inositol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | 96% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | pyruvate + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | 80% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate | Euphorbia characias | pyridoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | each subunit contain one Fe(III) and one Zn(II) ion. The two subunits are connected by a disulfide bridge. 2 * 68000, SDS-PAGE | Euphorbia characias |
Synonyms | Comment | Organism |
---|---|---|
ELPAP | - |
Euphorbia characias |
latex purple acid phosphatase | - |
Euphorbia characias |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
standard assay at | Euphorbia characias |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | enzyme is stable at 50°C but loses 70% of its activity at 60°C | Euphorbia characias |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
790 | - |
4-nitrophenyl phosphate | pH 5.75, 25°C | Euphorbia characias |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.75 | - |
- |
Euphorbia characias |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
vanadate | pH 5.75, 25°C | Euphorbia characias | |
0.0008 | - |
molybdate | pH 5.75, 25°C | Euphorbia characias |