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Literature summary for 3.1.3.2 extracted from

  • Singh, H.; Malinski, T.J.; Reilly, T.J.; Henzl, M.T.; Tanner, J.J.
    Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida (2011), Arch. Biochem. Biophys., 509, 76-81.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
the 1.85 A resolution crystal structure of the class C acid phosphatase from Pasteurella multocida is reported. The structure shows that rPmCCAP exhibits the same haloacid dehalogenase fold and dimeric assembly as the class C enzyme from Haemophilus influenzae Pasteurella multocida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
5'-AMP pH 5.5, 37°C Pasteurella multocida
0.29
-
NMN pH 5.5, 37°C Pasteurella multocida
0.5
-
4-nitrophenyl phosphate pH 5.5, 37°C Pasteurella multocida
0.8
-
3'-AMP pH 5.5, 37°C Pasteurella multocida
1.4
-
2'-AMP pH 5.5, 37°C Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Pasteurella multocida B9VWB2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-AMP + H2O
-
Pasteurella multocida adenosine + phosphate
-
?
3'-AMP + H2O
-
Pasteurella multocida adenosine + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Pasteurella multocida 4-nitrophenol + phosphate
-
?
5'-AMP + H2O
-
Pasteurella multocida adenosine + phosphate
-
?
NMN + H2O
-
Pasteurella multocida ?
-
?

Synonyms

Synonyms Comment Organism
acid phosphatase
-
Pasteurella multocida
rPmCCAP
-
Pasteurella multocida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pasteurella multocida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
2'-AMP pH 5.5, 37°C Pasteurella multocida
0.18
-
5'-AMP pH 5.5, 37°C Pasteurella multocida
0.19
-
3'-AMP pH 5.5, 37°C Pasteurella multocida
4.4
-
NMN pH 5.5, 37°C Pasteurella multocida
6.2
-
4-nitrophenyl phosphate pH 5.5, 37°C Pasteurella multocida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Pasteurella multocida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
2'-AMP pH 5.5, 37°C Pasteurella multocida
0.24
-
3'-AMP pH 5.5, 37°C Pasteurella multocida
2.3
-
5'-AMP pH 5.5, 37°C Pasteurella multocida
12
-
4-nitrophenyl phosphate pH 5.5, 37°C Pasteurella multocida
15
-
NMN pH 5.5, 37°C Pasteurella multocida